Humanin dosage and protocol
Humanin is a mitochondria-derived peptide with powerful cytoprotective, neuroprotective, and anti-aging effects. It protects neurons from amyloid-beta toxicity (Alzheimer's-related), improves insulin sensitivity, and has anti-inflammatory properties. Levels decline with age, making it a target for anti-aging research.
- Vial size: 5 mg
- Reconstitution: 1 mL bacteriostatic water
- Concentration: 5mg/mL
- Administration: subcutaneous
Research areas
- Anti-Aging
- Neuroprotection
- Metabolic Health
- Longevity
- Alzheimer's Research
Quick start
- Reconstitute: Add 1 mL bacteriostatic water to the 5 mg vial → 5 mg/mL
- Typical dose: 2-4 mg subcutaneously 2-3 times per week for 8-12 weeks
- Easy measuring: At 5 mg/mL, 1 unit = 0.01 mL = 50 mcg on a U-100 syringe, so 2 mg = 40 units and 4 mg = 80 units
- Storage: Lyophilized at -20°C; refrigerate at 2-8°C after mixing and use within 2-3 weeks
Dosing protocol
| Phase | Dose | Frequency | Volume |
|---|---|---|---|
| Standard | 2000-4000mcg (2-4mg) | 2-3x per week | 0.4-0.8mL |
How it works
Humanin is a 24-amino-acid peptide encoded within mitochondrial DNA, one of a small family of mitochondria-derived peptides. It acts both inside cells, where it binds pro-apoptotic proteins such as Bax and blocks cell-death signaling, and outside cells through receptors including a complex involving CNTFR, WSX-1 and gp130 (activating STAT3) and the formyl-peptide receptor FPRL1. Humanin was discovered in brain tissue that had been spared in Alzheimer's disease and protects neurons from amyloid-beta toxicity in cell culture. In rodent studies, humanin or its more potent analog HNG improved insulin sensitivity, reduced damage after heart and brain ischemia, and lessened age-related decline in some measures. Circulating levels fall with age in humans, and higher levels have been associated with longevity in some observational studies of long-lived families. There are no published clinical trials of humanin injections in humans, so effects on cognition, metabolism or aging in people are unknown. Most research uses analogs rather than native humanin, and doses in this record are extrapolated rather than derived from human studies.
Reconstitution steps
- Draw 1.0 mL bacteriostatic water with a sterile syringe
- Inject slowly down the vial wall; avoid foaming
- Gently swirl/roll until dissolved (do not shake)
- Label with reconstitution date and refrigerate at 2–8°C, protected from light; use within 2–3 weeks
Storage
Store lyophilized at −20°C (−4°F). After reconstitution, refrigerate at 2–8°C and use within 2–3 weeks.
Important notes
- Research use only
- Mitochondria-derived peptide — declines with age
- Neuroprotective against Alzheimer's-related amyloid-beta
- Improves insulin sensitivity
- Often stacked with MOTS-c for comprehensive anti-aging protocol
Supplies
- Bacteriostatic water
- Insulin syringes
- Alcohol swabs