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Kisspeptin-10 dosage and protocol
Puberty peptide that stimulates sex hormone production and enhances fertility in both men and women
- Vial size: 10 mg
- Reconstitution: 3 mL bacteriostatic water
- Concentration: 3.33 mg/mL
- Administration: subcutaneous
Research areas
- Testosterone Support
- Fertility
- Sexual Health
- Hormone Optimization
Quick start
- Reconstitute: Add 3 mL bacteriostatic water to the 5 mg vial → about 1.67 mg/mL
- Typical dose: 100-200 mcg, 3-5 times weekly for 4 weeks, then 4 weeks off
- Easy measuring: At 1.67 mg/mL, 1 unit = 0.01 mL ≈ 16.7 mcg on a U-100 syringe, so 100 mcg ≈ 6 units and 200 mcg ≈ 12 units
- Storage: Unreconstituted at -20°C; refrigerate after mixing
Dosing protocol
| Phase | Dose | Frequency | Volume |
|---|---|---|---|
| Weeks 1–2 | 100 mcg | Once daily (SC) | 0.03 mL (3 units) |
| Weeks 3–8 | 200 mcg | Once daily (SC) | 0.06 mL (6 units) |
How it works
Kisspeptin-10 is the shortest active form of kisspeptin, a family of peptides encoded by the KISS1 gene. It activates the kisspeptin receptor (KISS1R, formerly GPR54) on gonadotropin-releasing hormone (GnRH) neurons in the hypothalamus. This triggers GnRH release, which in turn prompts the pituitary to release luteinizing hormone (LH) and, to a lesser extent, follicle-stimulating hormone (FSH). LH and FSH then drive testosterone production and sperm development in men, and ovarian hormone production and ovulation in women. Loss-of-function mutations in the receptor cause failure of puberty, which is how the system's importance was discovered. In small human clinical studies, injected kisspeptin-10 rapidly raised LH and testosterone in healthy men, and it has been studied in people with low reproductive hormone levels. The longer form, kisspeptin-54, has been used experimentally to trigger egg maturation in IVF trials with a reportedly lower risk of ovarian hyperstimulation than standard triggers. Kisspeptin-10 has a very short half-life (minutes), and continuous exposure can desensitize the system and suppress, rather than stimulate, hormone release. It is not an approved medicine, and evidence is limited to short-term research studies.
Reconstitution steps
- Allow to room temperature
- Add 3mL bacteriostatic water
- Swirl gently
- Refrigerate
Storage
Store unreconstituted at -20°C. Refrigerate after mixing
Important notes
- Stimulates GnRH release in hypothalamus
- Releases LH and FSH in pituitary
- Men: LH/FSH signal testes to produce testosterone and sperm
- Women: LH/FSH signal ovaries to produce estrogen and trigger ovulation
- 1mcg per kg body weight (100-200mcg typical)
- Best in fasted state in morning for natural testosterone rhythm
- Peak FSH & LH occurs 45-60 minutes after injection
- 3-5x week, 1 month on, 1 month off
- For fertility: take 45-60 mins before conception attempts
- Temporarily increases oxytocin
- Low cost with high benefit profile
Supplies
- Bacteriostatic water (5mL)
- Insulin syringes (0.5mL)
- Alcohol swabs