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Testagen (20 mg Vial) dosage and protocol

Testagen is a tetrapeptide (Lys-Asp-Glu-Gln) bioregulator derived from testicular tissue, part of the Khavinson peptide family. Research focuses on its role in supporting testicular function, Leydig cell activity, and endogenous testosterone synthesis through epigenetic modulation of gonadal tissue. May be used to support testosterone production and male reproductive health.

  • Vial size: 20 mg
  • Reconstitution: 3 mL bacteriostatic water
  • Concentration: 6.67 mg/mL
  • Administration: subcutaneous

Research areas

  • Testosterone Support
  • Testicular Function
  • Leydig Cell Activity
  • Male Reproductive Health
  • Hormone Optimization

Quick start

  • Reconstitute: Add 3.0mL bacteriostatic water → ~6.67mg/mL concentration
  • Typical dose: 100–200mcg per injection, 5x weekly for 4–6 weeks
  • Storage: Lyophilized at −20°C; reconstituted refrigerated at 2–8°C; use within 4 weeks

Dosing protocol

PhaseDoseFrequencyVolume
Weeks 1–2100mcg5x weekly~1.5 units (0.015mL)
Weeks 3–6100–200mcg5x weekly~1.5–3 units (0.015–0.03mL)

How it works

Testagen interacts with chromatin in testicular Leydig cells, activating gene expression pathways involved in steroidogenesis and testosterone biosynthesis. As a Khavinson bioregulator, it works through epigenetic mechanisms to restore or maintain normal cellular function in gonadal tissue, particularly beneficial when testicular function is suppressed.

Reconstitution steps

  • Draw 3.0mL bacteriostatic water with a sterile syringe
  • Inject slowly down the vial wall; avoid foaming
  • Gently swirl/roll until dissolved (do not shake)
  • Label concentration (~6.67mg/mL) and date; refrigerate at 2–8°C

Storage

Lyophilized: store at −20°C. After reconstitution, refrigerate at 2–8°C and use within 4 weeks.

Important notes

  • Research use only — Khavinson peptide class
  • Monitor total and free testosterone levels to track response
  • Can be combined with Gonadorelin or Kisspeptin for HPG axis support
  • Not a replacement for TRT in cases of primary hypogonadism
  • Rotate injection sites to prevent irritation

Supplies

  • Testagen vial (20mg)
  • Bacteriostatic water (10mL)
  • Insulin syringes (U-100, 50-unit)
  • Alcohol swabs
  • Sharps container

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Calculate your Testagen (20 mg Vial) dose in syringe units

Crestigen is an educational research reference. Nothing here is medical advice; many of these compounds are not approved for human use. Talk to a licensed clinician before using any substance.